Summary
Objective
Carboxymethyllysine (CML) and homocitrulline (HCit) are the products of two non-enzymatic
post-translational modifications of protein, a process related to age. We investigated
whether serum CML and HCit concentrations were associated with hand osteoarthritis
(HOA), especially erosive HOA.
Design
Serum CML and HCit were measured by using liquid chromatography coupled with tandem
mass spectrometry at inclusion in 386 patients included in the DIGItal Cohort Design
(DIGICOD) cohort. We investigated whether serum CML and/or HCit concentrations were
associated with erosive HOA or with HOA clinical and radiological features. Moreover,
we compared the tissular concentrations of CML and HCit in OA and non-OA cartilage
from proximal interphalangeal and metacarpo-phalangeal (MCP) joints from human cadaveric
donors.
Results
Median (IQR) serum CML concentration was lower in patients with erosive HOA than those
with non-erosive HOA (178.7 [157.1–208.8] vs 194.7 [168.9–217.1] μmol/mol Lys, P = 0.002), but median HCit concentration did not differ between the groups (193.9
[162.9–232.0] vs 193.9 [155.9–224.6] μmol/mol Lys). Cartilage HCit and CML concentrations
were not correlated with clinical features. Serum CML concentration was higher in
OA than non-OA MCPs (7.0 vs 4.0 mmol/mol Lys, P = 0.01).
Conclusions
Serum CML concentration was lower in erosive HOA than non-erosive HOA, and cartilage
CML concentration was higher in OA than non-OA cartilage. These results encourage
further studies to test whether serum CML could be a new prognostic biomarker in HOA.
Keywords
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Article info
Publication history
Published online: March 14, 2023
Accepted:
March 3,
2023
Received:
August 8,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2023 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
