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Circulating miRNAs in hand osteoarthritis

  • Author Footnotes
    a Jiří Baloun and Aneta Pekáčová contributed equally to the work.
    J. Baloun
    Correspondence
    Address correspondence and reprint requests to: J. Baloun, Institute of Rheumatology, 128 00 Prague, Czech Republic. Tel: 420-234-075-329.
    Footnotes
    a Jiří Baloun and Aneta Pekáčová contributed equally to the work.
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
    Search for articles by this author
  • Author Footnotes
    a Jiří Baloun and Aneta Pekáčová contributed equally to the work.
    A. Pekáčová
    Footnotes
    a Jiří Baloun and Aneta Pekáčová contributed equally to the work.
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • X. Švec
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • T. Kropáčková
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • V. Horvathová
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • H. Hulejová
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic
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  • K. Prajzlerová
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic
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  • O. Růžičková
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • O. Šléglová
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • J. Gatterová
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • M. Tomčík
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • M. Filková
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • J. Vencovský
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • K. Pavelka
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • L. Šenolt
    Affiliations
    Institute of Rheumatology, 128 00 Prague, Czech Republic

    Department of Rheumatology, 1st Faculty of Medicine, Charles University, 128 00 Prague, Czech Republic
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  • Author Footnotes
    a Jiří Baloun and Aneta Pekáčová contributed equally to the work.
Published:November 12, 2022DOI:https://doi.org/10.1016/j.joca.2022.10.021

      Summary

      Objective

      Hand osteoarthritis (OA) is a frequent musculoskeletal disorder with an increasing prevalence during ageing. This study aimed to evaluate circulating microRNAs (miRNAs) in the plasma of patients with hand OA compared with age- and sex-matched healthy control subjects.

      Methods

      In total, 238 participants (96 with erosive and 73 with non-erosive hand OA patients and 69 healthy control subjects) were included in this study. All patients underwent clinical examinations, including self-reported measures (AUSCAN and Algofunctional index). Radiographs of both hands were scored with the Kallman scale. The profile of miRNAs in plasma was screened using TaqMan™ Low-Density Array, and candidate miRNAs were validated on two quantitative real-time PCR (qRT-PCR) systems (QuantStudio and SmartChip).

      Results

      Of all the 754 miRNAs, 40 miRNAs were different between hand OA patients and healthy control subjects in the screening cohort. Following the two-phase validation process, three miRNAs (miR-23a-3p, miR-146a-5p, and miR-652-3p) were increased in patients with hand OA compared with healthy control subjects and were associated with the AUSCAN sum score and AUSCAN pain. Furthermore, an inverse correlation of miR-222-3p with the Kallman radiographic score was found. The expression of miRNAs did not differ between erosive and non-erosive hand OA.

      Conclusion

      The profile of circulating miRNAs could unveil candidate biomarkers associated with hand OA symptoms. Longitudinal studies are required to determine the role of miRNAs in hand OA.

      Keywords

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