Synovial fluid CD34− CD44+ CD90+ mesenchymal stem cell levels are associated with the severity of primary knee osteoarthritis

Lee, D.-H.; Sonn, C.H.; Han, S.-B.; Oh, Y.; Lee, K.-M.; Lee, S.-H.

doi:10.1016/j.joca.2011.11.010

« Previous Next »Osteoarthritis and Cartilage
Volume 20, Issue 2 , Pages 106-109, February 2012

Synovial fluid CD34⁻ CD44⁺ CD90⁺ mesenchymal stem cell levels are associated with the severity of primary knee osteoarthritis

D.-H. Lee
- Department of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Republic of Korea
- These authors contributed equally to this manuscript.
C.H. Sonn
- Department of Biochemistry and Molecular Biology, Division of Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Seoul, Republic of Korea
- These authors contributed equally to this manuscript.
S.-B. Han
- Department of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Republic of Korea
Y. Oh
- Department of Biochemistry and Molecular Biology, Division of Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Seoul, Republic of Korea
K.-M. Lee
- Department of Biochemistry and Molecular Biology, Division of Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Seoul, Republic of Korea
- Address correspondence and reprint requests to: K.-M. Lee, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, 126-1, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-701, Republic of Korea. Tel: 82-2-920-6253; Fax: 82-2-920-6252.
S.-H. Lee
- Department of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Republic of Korea
- Address correspondence and reprint requests to: S.-H. Lee, Department of Orthopaedic Surgery, Korea University Anam Hospital, Korea University College of Medicine, 126-1, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-705, Republic of Korea. Tel: 82-2-920-5924; Fax: 82-2-924-2471.

Received 24 June 2011; accepted 18 November 2011. published online 19 December 2011.

Summary

To the best of our knowledge, no reports have directly compared synovial fluid (SF)- and synovial membrane (SM)-derived mesenchymal stem cells (MSCs) from primary knee osteoarthritis patients in terms of MSC proportion, either immediately after isolation or during culture. Any possible correlation between SM- and SF-MSC purity and osteoarthritis severity, also remains unclear. We therefore assessed quantitative and phenotypic differences in MSCs isolated from SF and SM. We also evaluated the correlation between sample MSC purity, and disease severity, in patients with osteoarthritis. The main result of the current study was that the mean SF-MSC proportion at passage 0 was negatively correlated with Kellgren–Lawrence (KL) grade (r = −0.565, P = 0.002). In addition, KL grade was a only significant independent negative predictor of SF-MSC proportion at passage 0 (β = −0.356, P = 0.039). Conclusively, the proportion of SF-MSCs in fresh samples, evaluated at the single cell level, was inversely correlated with osteoarthritis severity.

Keywords: Synovium, Synovial fluid, Osteoarthritis, Mesenchymal stem cell