Osteoarthritis and Cartilage
Volume 18, Issue 9 , Pages 1167-1173, September 2010

Hypoxia, RONS and energy metabolism in articular cartilage

  • B. Fermor

      Affiliations

    • Corresponding Author InformationAddress correspondence and reprint requests to: Beverley Fermor, Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Box 3093, 375 Medical Sciences Research Building, Durham, NC 27710, USA. Tel: 1-919-681-6867; Fax: 1-919-684-8490.
    • ,
  • A. Gurumurthy
    • ,
  • B.O. Diekman

Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA

Received 6 January 2010; accepted 9 June 2010. published online 14 July 2010.

Summary 

Objective

Increased pro-inflammatory cytokines and reactive oxygen and nitrogen species (RONS) occur in osteoarthritis (OA). Oxygen tension can alter the levels of RONS induced by interleukin-1 (IL-1). RONS such as nitric oxide (NO) can alter energy metabolism. The aim of this study was to determine if oxygen tension alters energy metabolism, in articular cartilage, in response to IL-1 or NO and to determine if cell death occurred.

Design

Porcine articular chondrocytes were incubated with IL-1 or the NO donor NOC-18 for 48h in either 1, 5 or 20% O2. Adenosine triphosphate (ATP) levels were measured and immunoblots for adenosine monophosphate-activated protein kinase (AMPK) were done. Protein translation was measured by S6 activation. Senescence and autophagy were determined by increased caveolin or conversion of LC3-I to LC3-II respectively.

Results

One percent O2 significantly reduced ATP levels compared with 20% O2. Five percent O2 significantly increased ATP levels compared with 20% O2. One percent O2 significantly increased phospho-AMPK (pAMPK) protein expression compared with 5 or 20% O2. Oxygen tension had no effects on pS6, caveolin or LC3-II levels. IL-1-induced NO production was significantly reduced with decreased oxygen tension, and significantly reduced ATP levels at all oxygen tensions, but pAMPK was only significantly increased at 5% O2. IL-1 significantly reduced pS6 at all oxygen tensions. IL-1 had no effects on caveolin and significantly increased LC3-II at 20% O2 only. NOC-18 significantly reduced ATP levels at all oxygen tensions, and significantly increased pAMPK at 5% O2 only, and significantly decreased pAMPK at 1% O2. NOC-18 significantly reduced pS6 at 1% O2 and significantly increased caveolin at 5% O2, and LC3-II at 1% O2.

Conclusion

Our data suggest 5% O2 is optimal for energy metabolism and protective to some effects of IL-1 and NO. NO has the greatest effects on ATP levels and the induction of autophagy at 1% O2.

Keywords: Articular cartilage, Oxygen tension, ATP, Interleukin 1, Senescence, Autophagy

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PII: S1063-4584(10)00202-5

doi:10.1016/j.joca.2010.06.004

Osteoarthritis and Cartilage
Volume 18, Issue 9 , Pages 1167-1173, September 2010

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