Osteoarthritis and Cartilage
Volume 18, Issue 1 , Pages 126-137 , January 2010

Mechanical loading regimes affect the anabolic and catabolic activities by chondrocytes encapsulated in PEG hydrogels

  • G.D. Nicodemus
    • ,
  • S.J. Bryant

      Affiliations

    • Corresponding Author InformationAddress correspondence and reprint requests to: S. J. Bryant, Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309, USA. Tel: 1-303-735-6714; Fax: 1-303-492-4341.

Received 6 March 2009 ,Accepted 20 August 2009.

References 

  1. Guilak F, Butler DL, Goldstein SA. Functional tissue engineering – the role of biomechanics in articular cartilage repair. Clin Orthop Relat Res. 2001;S295–S305
  2. Hung CT, Mauck RL, Wang CCB, Lima EG, Ateshian GA. A paradigm for functional tissue engineering of articular cartilage via applied physiologic deformational loading. Ann Biomed Eng. 2004;32:35–49
  3. Mauck RL, Byers BA, Yuan X, Tuan RS. Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading. Biomech Model Mechanobiol. 2007;6:113–125
  4. Chung C, Erickson IE, Mauck RL, Burdick JA. Differential behavior of auricular and articular chondrocytes in hyaluronic acid hydrogels. Tissue Eng Part A. 2008;14:1121–1131
  5. Clark IM, Murphy G. Matrix proteinases. In:  Seibel MJ,  Robins SP,  Bilezikian JP editor. Dynamics of Bone and Cartilage Metabolism. Burlington: Elsevier; 2006;p. 181–198
  6. Manicourt DH, Devogelaer JP, Thonar EJ. Products of cartilage metabolism. In:  Seibel MJ,  Robins SP,  Bilezikian JP editor. Dynamics of Bone and Cartilage Metabolism. Burlington: Elsevier; 2006;p. 421–449
  7. Bau B, Gebhard PM, Haag J, Knorr T, Bartnik E, Aigner T. Relative messenger RNA expression profiling of collagenases and aggrecanases in human articular chondrocytes in vivo and in vitro. Arthritis Rheum. 2002;46:2648–2657
  8. Kevorkian L, Young DA, Darrah C, Donell ST, Shepstone L, Porter S, et al. Expression profiling of metalloproteinases and their inhibitors in cartilage. Arthritis Rheum. 2004;50:131–141
  9. De Croos JNA, Dhaliwal SS, Grynpas MD, Pilliar RM, Kandel RA. Cyclic compressive mechanical stimulation induces sequential catabolic and anabolic gene changes in chondrocytes resulting in increased extracellular matrix accumulation. Matrix Biol. 2006;25:323–331
  10. Bryant SJ, Anseth KS. Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels. J Biomed Mater Res. 2002;59:63–72
  11. Bryant SJ, Bender RJ, Durand KL, Anseth KS. Encapsulating chondrocytes in degrading PEG hydrogels with high modulus: engineering gel structural changes to facilitate cartilaginous tissue production. Biotechnol Bioeng. 2004;86:747–755
  12. Elisseeff J. Injectable cartilage tissue engineering. Expert Opin Biol Ther. 2004;4:1849–1859
  13. Nicodemus GD, Bryant SJ. The role of hydrogel structure and dynamic loading on chondrocyte gene expression and matrix formation. J Biomech. 2008;41:1528–1536
  14. Villanueva I, Hauschulz DS, Mejic D, Bryant SJ. Static and dynamic compressive strains influence nitric oxide production and chondrocyte bioactivity when encapsulated in PEG hydrogels of different crosslinking densities. Osteoarthritis Cartilage. 2008;16:909–918
  15. Chowdhury TT, Bader DL, Shelton JC, Lee DA. Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression. Arch Biochem Biophys. 2003;417:105–111
  16. Kelly TAN, Ng KW, Wang CCB, Ateshian GA, Hung CT. Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures. J Biomech. 2006;39:1489–1497
  17. Kisiday JD, Jin MS, DiMicco MA, Kurz B, Grodzinsky AJ. Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffolds. J Biomech. 2004;37:595–604
  18. Mauck RL, Seyhan SL, Ateshian GA, Hung CT. Influence of seeding density and dynamic deformational loading on the developing structure/function relationships of chondrocyte-seeded agarose hydrogels. Ann Biomed Eng. 2002;30:1046–1056
  19. Mauck RL, Soltz MA, Wang CCB, Wong DD, Chao PHG, Valhmu WB, et al. Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. ASME J Biomech Eng. 2000;122:252–260
  20. Waldman SD, Couto DC, Grynpas MD, Pilliar RM, Kandel RA. A single application of cyclic loading can accelerate matrix deposition and enhance the properties of tissue-engineered cartilage. Osteoarthritis Cartilage. 2006;14:323–330
  21. Lima EG, Bian L, Ng KW, Mauck RL, Ba Byers, Tuan RS, et al. The beneficial effect of delayed compressive loading on tissue-engineered cartilage constructs cultured with TGF-beta 3. Osteoarthritis Cartilage. 2007;15:1025–1033
  22. Anseth KS, Metters AT, Bryant SJ, Martens PJ, Elisseeff JH, Bowman CN. In situ forming degradable networks and their application in tissue engineering and drug delivery. J Control Release. 2002;78:199–209
  23. Bryant SJ, Anseth KS. Controlling the spatial distribution of ECM components in degradable PEG hydrogels for tissue engineering cartilage. J Biomed Mater Res A. 2003;64A:70–79
  24. Bryant SJ, Durand KL, Anseth KS. Manipulations in hydrogel chemistry control photoencapsulated chondrocyte behavior and their extracellular matrix production. J Biomed Mater Res A. 2003;67A:1430–1436
  25. Sawhney AS, Pathak CP, Hubbell JA. Bioerodible hydrogels based on photopolymerized poly(ethylene glycol)-co-poly(alpha-hydroxy acid) diacrylate macromers. Macromolecules. 1993;26:581–587
  26. Bryant SJ, Nuttelman CR, Anseth KS. Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro. J Biomater Sci Polym Ed. 2000;11:439–457
  27. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques. 1993;15:532
  28. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29:
  29. Farndale RW, Buttle DJ, Barrett AJ. Improved quantitation and discrimination of sulfated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta. 1986;883:173–177
  30. Davisson T, Kunig S, Chen A, Sah R, Ratcliffe A. Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage. J Orthop Res. 2002;20:842–848
  31. Kisiday JD, Lee JH, Siparsky PN, Frisbie DD, Flannery CR, Sandy JD, et al. Catabolic responses of chondrocyte-seeded peptide hydrogel to dynamic compression. Ann Biomed Eng. 2009;37:1368–1375
  32. Blain EJ. Mechanical regulation of matrix metalloproteinases. Front Biosci. 2007;12:507–527
  33. Guilak F, Alexopoulos LG, Upton ML, Youn I, Choi JB, Cao L, et al. The pericellular matrix as a transducer of biomechanical and biochemical signals in articular cartilage. Skeletal Development and Remodeling in Health, Disease, and Aging. 2006;1068:498–512
  34. Poole CA. Articular cartilage chondrons: form, function and failure. J Anat. 1997;191:1–13
  35. Bryant SJ, Chowdhury TT, Lee DA, Bader DL, Anseth KS. Crosslinking density influences chondrocyte metabolism in dynamically loaded photocrosslinked poly(ethylene glycol) hydrogels. Ann Biomed Eng. 2004;32:407–417
  36. Bryant SJ, Anseth KS, Lee DA, Bader DL. Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain. J Orthop Res. 2004;22:1143–1149
  37. Grodzinsky AJ, Levenston ME, Jin M, Frank EH. Cartilage tissue remodeling in response to mechanical forces. Annu Rev Biomed Eng. 2000;2:691–713
  38. Quinn TM, Grodzinsky AJ, Buschmann MD, Kim YJ, Hunziker EB. Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants. J Cell Sci. 1998;111:573–583
  39. Sah RLY, Kim YJ, Doong JYH, Grodzinsky AJ, Plaas AHK, Sandy JD. Biosynthetic response of cartilage explants to dynamic compression. J Orthop Res. 1989;7:619–636
  40. Pufe T, Lemke A, Kurz B, Petersen W, Tillmann B, Grodzinsky AJ, et al. Mechanical overload induces VEGF in cartilage discs via hypoxia-inducible factor. Am J Pathol. 2004;164:185–192
  41. Buschmann MD, Kim YJ, Wong M, Frank E, Hunziker EB, Grodzinsky AJ. Stimulation of aggrecan synthesis in cartilage explants by cyclic loading is localized to regions of high interstitial fluid flow. Arch Biochem Biophys. 1999;366:1–7
  42. Evans RC, Quinn TM. Dynamic compression augments interstitial transport of a glucose-like solute in articular cartilage. Biophys J. 2006;91:1541–1547
  43. Garcia AM, Frank EH, Grimshaw PE, Grodzinsky AJ. Contributions of fluid convection and electrical migration to transport in cartilage: relevance to loading. Arch Biochem Biophys. 1996;333:317–325
  44. Hardingham T. Proteoglycans and glycosaminoglycans. In:  Seibel MJ,  Robins SP,  Bilezikian JP editor. Dynamics of Bone and Cartilage Metabolism. Burlington: Elsevier; 2006;p. 85

PII: S1063-4584(09)00211-8

doi: 10.1016/j.joca.2009.08.005

Osteoarthritis and Cartilage
Volume 18, Issue 1 , Pages 126-137 , January 2010

Article Tools

* Image Usage & Resolution